1. Tip Artifacts


1.3 Blunt Tip


This artifact is common after scanning for a long time with the same tip. Tips may last very long, or sometimes go blunt quickly. Some samples (known as "tip-eaters") have a tendency to lead to blunt tips very quickly.
In order to find out if your tip is blunt, scan a well-known sample like this one. See the list of tip-characterisation samples.

AFM image showing blunt tip

In the example above, the image on the right is made with a blunt tip, the left with a sharp (new) one.

How to avoid it
Use your tips carefully! If it gets blunt, replace it! In general, scanning with a lower applied force (see set-point) helps to reduce tip wear.

Some low-wear tips which can scan for longer before becoming blunt are also available.

1. Tip Effects

1.4 Double/multiple tips


if the tip is broken, or contaminated, you can often get a "double tip" effect, where both the tip and the contamination are scanning the surface.
See the example below.

AFM image double tip

In the example above, each DNA molecule has a "twin", caused by the double tip.


How to avoid it
You can check that this is occurring with a tip characterisation sample. If it happens, change the tip. Once a tip is broken, you must change it. If it is contamination, you MIGHT be able to clean it. But most often, even in this case, you will have to change to a new tip.

 

 

2. Scanner Artifacts

2.2 Edge Overshoot



This artifact occurs when the scanner moves further than it should vertically, leading to a very "sharp" edge on the features in the image.
You can spot it in the topography profile as "bumps" on the edges of tall features. It's due to hysteresis in piezoelectric scanners.


How to avoid it.
If you scan more slowly, you will avoid this artifact. However, it is usually only noticable on samples that are very flat, with square features.

2. Scanner Artifacts

 

2.1 Piezo Creep


Piezo creep occurs because when you apply a set voltage to the piezo(scanner), and then try to maintain it, in order to move to a certain location, the piezo tends to continue to move in the same direction for a certain period of time. Essentially, this means that when you start a new scan, you will get some stretching or compression of features at the beginnning of the image, and then the image will start to appear more normal over time.
The effect will be particularly pronounced if you move to a new place within your scan range.
See the example below.

AFM image showing piezo or scanner creep artifact

In the example above, the image is distorted at the top due to scanner creep.

How to avoid it
The simplest thing to do is just wait until it disappears, and start scanning again. Even better, set the AFM to scan one line continuously. Select the first line of the area you wish to scan, and wait until the image stabilises (i.e. that line is always the same), then start scanning the image properly.
AFMs with linearised scanner do not suffer from this effect. many modern AFMs have such scan linearising features. More details about his can be found in the book "Atomic Force Microscopy".
Note that distorted features all over your image, not just at the start, are due to something else, maybe sample drift.


2. Other Artifacts


Sample Drift


Sample drift means that your sample is moving as you try to scan it in the AFM.
This artifact occurs in any type of SPM, or indeed any microscopy, but is much more serious in high resolution techniques like SEM, TEM and AFM than optical microscopy. It's known as "drift", because, in general, we see the sample moving slowly, in one direction (or expanding or contracting). You can generally recognize this as a "distortion" of the image, which changes when you change the slow scan direction. beginning of the image, and then the image will start to appear more normal over time. The effect will be particularly pronounced if you move to a new place within your scan range.
See the example below.

Two AFM images showing effects of sample drift

In the example above, the two images of the same cluster of bacteria are not identical because the sample is moving while it is being scanned. It is typical of this effect that you will get different images when scanning in different directions.

How to avoid it.
The best thing to do, is to stop your sample from moving. The way to do this depends on why it's moving.

Is it thermal expansion?
-then keep the temperature constant, turn off external sources of heat(lights), and wait for it to stop moving.
It's usually worth trying to fix the sample down better. The other way to overcome (or at least to reduce) the distortion is to scan quickly.

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