Approach (Vibrating Mode)
- Select “Vibrating”
- Ensure the Range Check is Complete (with green light) before approaching. DO range check with the probe somewhat close to the sample, so that you can see both sample and probe when range check is going on. During range check, the sample should translate around a square under the probe. If this does not happen, there is a problem.
- Moving the probe towards the sample is achieved with the Manual Z motor control buttons, “Up” and “Down”. It is recommended to use a speed less than half the maximum in this control. The best way to tell if the probe is approaching your sample, is to focus on the sample, and move the probe down until it is NEARLY in focus.
- Laser should already be aligned on probe, the place of the red dot on the photodetector is not very important. Ideally, TB should be in the middle of its range.
- The “vibrating” option makes the “Tune Frequency” window active.
- Use this window to find the resonant frequency of the probe, and set the correct amplitude:
- You should know the range of possible values of the natural resonant frequency (Rf) of your probe, if not, look on the box. For AppNano ACT probes, it-s 200-400kHz.
- Set “Lower” and “Upper” values accordingly to sweep this range. Note It won’t work if you have a “selected” value outside of the range.
- An initial guess for the driving amplitude (“Amplitude Vpp”), would be 0.4. You need at least 1 step per KHz, so for the above case, set steps to 200.
- Press “Sweep”
- You should see a sharp positive peak in the amplitude trace somewhere in that range (fig 4a). Move the green (lower), blue (selected) and red (upper) lines to close in on the peak. DO this by putting the green line on the left og the peak, the blue on in the middle of it and the red one on the right of the peak. Reduce the number of steps. Typically 50 steps would be a good choice.
- Repeat a sweep, with fewer steps (e.g. 50). Do this until the peak fills most to the windows as in figure 4b.
FIGURE 4 Tune window: A: Initial tune; B: Tune at scale suitable for selecting operating conditions.
- When you can see the peak clearly as in figure 4b adjust the Driving Amplitude (Vpp) to achieve the desired peak amplitude in the window, for example 1.0V.
- Now, the operating frequency should be chosen. Ideally, you will do this with the probe already close to the sample surface. If necessary, use “Down” button to achieve this, using the focus of the video microscope to help you get close.
- This guide assumes you use software version 1.5.6. Other versions will be different in this part of the procedure.
- Put the blue line on the highest point of the amplitude curve.
- Click system tab. Look at the amplitude value, it should be oscillating a little in the decimal points. Note the value.
- Go back to pre-scan window and move the blue line a little to the left. Check the amplitude value in system again. IF the amplitude increase, move a bit to the left again, and re-check. You want the amplitude to decrease by 2-5 % on moving left, compared to the maximum value. For example, if you have a maximum of 1.00V, look for a value of 0.95-0.98 V.
- NOTE: This procedure is particularly confusing because of a bug in the software, which means the REAL amplitude curve is shifted to the left of the DISPLAYED amplitude curve by ca.100Hz.
- Once you’ve done this, click “Lock Off”, which will turn to “Lock On”.
- Amplitude value will no longer oscillate.
- Ideally, you should start with a maximum amplitude between 1 and 1.2 V.
- Before automated tip approach you should get the probe as close to the sample as possible, without risking damage to the probe.
- Press “start” in the “automated tip approach” box.
- The approach occurs step by step , in the “woodpecker” method (see [i])
- Eventually, it should say feedback ON (green light).Ideally z drive should be in the centre of the range (0 V).
- If not in centre, press start again, and see if it goes to centre, if necessary try again.
- If something goes wrong with approach, it's possible the procedure will press the tip HARD against the sample, so it's always a good idea to watch the video microscope while approach is occurring. If you see cantilever bending, STOP, and raise the tip.
[i] Atomic Force Microscopy, Eaton and West, Chapter 2.
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The Requimte AFM Training Workshop will run during Easter 2014, from the 14th to 17th April. Following the successful courses that ran in 2011 and 2013, we've decided the course should run annually. The course includes several hours hands-on training in acquiring images with the atomic force microscope as well as AFM data processing. The course has been reorganised based on student feedback, and will feature advanced topics lectures from guest scientists in biology and materials science. This year, we plan to lengthen the practical part of the course, and hope to offer access to different instruments.
- We are pleased to announce that this year, there will be 3 invited speakers, covering applications in a wide range of areas, and illustrating different capabilities of AFM:
Dr. Rui Rocha, CEMUP, Porto: "Materials Applications of AFM"
Dr. Simon Connell, University of Leeds, UK: "Dynamics in Biological AFM"
Dr. Filomena Carvalho, IMM, University of Lisbon, "Force Spectroscopy: Biological and Biomedical Applications"
Thanks to all the speakers for agreeing to talk.
- The provisional timetable can be downloaded here.
- There is a document containing in formation on travel to Porto, and hotels near the faculty of sciences here.
- The 2014 course has had all 16 places filled.
- The course flyer can be seen by clicking below:
A blog with information and student feedback from the 2013 course can be seen here: Requimte AFM Workshop 2013
Some information about the course that took place in 2011 can be seen here: http://atomicforceblog.blogspot.com/2011/09/2011-requimte-afm-mini-course.html
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In our lab, we have a TT-AFM, and an LS-AFM from AFM Workshop. We had one of the very first examples of this microscope, so although it's fairly new, the instrument, and the software has developed a lot during that time. On this page, I have collected together various materials that can help use the TT-AFM instrument. Note that although this material is all based on the TT-AFM, a lot of it probably applies to the other instruments from AFM Workshop, since they are all based on the same electronics. Lots of more general information about AFM operation can be found around this website (links on the left), and of course in my book.
UPDATE: The latest version of the AFM Workshop software 2.03, seems to be far superior to 1.58, which I was using previously. I reccommend strongly that everyone check this version out.
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This page contains currently a very small selection of the AFM images I have collected over the years. Please check back later to see more.
All images copyright 2010-2016, Peter Eaton, and may not be re-used without my permission
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"I really enjoyed my time on the course. In a simple and practical manner, I learned how to obtain results from AFM. I entered the course without ever being on the same lab with an AFM and left being able to carry out an experiment and analysing my own results."
The students enjoyed the course a lot, and we got lots of very positive feedback.
"I have to tell you that I'm enjoying a lot this mini-course. The language used to explain the concepts is very clear and there is a good atmosphere between all participants."
"I found it useful even for people (like me) having a certain experience in the field. For instance, things that work well in/with AFM can be easily found in a lot of papers, but things that are hard or tricky to set up are seldom explicitly written in articles. The workshop addressed specifically this type of issues. Also, to my opinion, attending the workshop was not expensive at all. "
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