Atomic Force Microscopy (2010), 256 pp, OUP. ISBN: 9780199570454
"Atomic Force Microscopy by Peter Eaton and Paul West is the manual that should accompany any AFM." - Prof. Othmar Marti, University of Ulm
"This classic text was the starting point from which the website afmhelp.com emerged, run and regularly updated by author Peter Eaton, with the explicit objective to contain all the AFM related material they couldn’t fit into the book! There’s a lot of love for the website from the AFM Community and wholesale agreement that the book behind it is a must-have too." - Nunano.com
Peter Eaton and Paul West share a common passion for atomic force microscopy. However, their involvement with atomic force microscopes are from very different perspectives. Over the past 10 years Peter used AFM's as the focal point of his research in a variety of scientific projects from materials science to biology. Paul, on the other hand, is an instrument builder and has spent the past 25 years creating microscopes for scientist and engineers. Together Peter and Paul have created an insightful book on the theory, practice and applications of atomic force microscopes. This book serves as an introduction to scientists and engineers that want to learn about these fascinating devices, and as a reference book for expert AFM users.
The Oxford University Press page describing the book can be found here, although the contents listed there are out of date. A correct contents overview can be found here: Book Contents.
The book was published on the 25th March 2010. There is also a Kindle Version of the book available. If you are affiliated to subscribing institution, then you may be able to access it via Oxford Scholarship Online.
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I've recently updated the SPM software list, with a couple of new (to me) programs, after many years! The links to the programs can be found on the software page.
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We will be running a two-day AFM course at The Bridge, at the University of Lincoln in June. Details can be found on the page linked below. You can also email me directly with questions.
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A request for help!
I am currently looking for one manual, and one piece of AFM control software:
- Topometrix Discoverer manual
- Nanocockpit software disc (CD-ROM?) This is the software to control a Nano-R AFM or similar from Pacific Nanotechology.
If you haveeither of these, please get in touch at This email address is being protected from spambots. You need JavaScript enabled to view it.
This page is intended to act as a repository for manuals for atomic force microscopes. At this time, there is relatively little information here (basically, the manuals I have .pdf copies of), but I hope that over time it will grow. If you have a manual for your instrument that you think could be useful for other AFM users, please send it to me at This email address is being protected from spambots. You need JavaScript enabled to view it..
I hope that this page is useful for AFM users, especially those who inherited older instruments that have become spearated.
AFMWorkshop
- TT2-AFM manual v2.3 - This .pdf file is the user manual for AFMWorkshop's 2nd gen TT-AFM , i.e. "Table-Top AFM"
- LS-AFM Manual v1.1 - This user manual is for AFMWorkshop's "Life Sciences AFM". This covers the AFM instrument, there may be a separate manual for the optical microscope from AFM Workshop if you have a copy of that one, please send it to me
- HR-AFM Manual - This .pdf file is the user manual for AFMWorkshop's "High Resolution AFM" instrument. Unlike the TT2 manual, it covers use of the v4.x software
- B-AFM Manual v1.0 - This is the user maual for the B-AFM from AFMWorkshop
Asylum Research (now Oxford Instruments)
- MFP 3D User Guide V13 - This is the .pdf file of the user guide for the MFP 3D Bio-AFM from Asylum which was since aquired by Oxford Instruments
- Cypher User Guide - This is the User guide for the Cypher "small sample AFM", from Asylum
Nanonics
- NWS User guide - This is the .pdf file of the user g uide for the NWS (NanoWorkshop) softwar euse dby Nanonics
- MV4000 user guide - This is the user guide (pdf) for theNanonics multiview 4000 AFM/SPM system. These systems are very unusual in supporting multiple probes scanning the sample at the same time (up to four I think).
DI/Veeco/Bruker
- Nanoscope Software 6.13 User Guide - This .pdf file is the user manual for the nanoscope software interface for any microscope using a nanoscope controller, i.e. the popular multimode and dimension instruments. This version is compatible with Windows XP latest.
- Multimode Instruction Manual v4.31 - This is a .pdf version of the instruction manual for the multimode microscope. This is based on a rather old version of the software (in DOS windows). Note that for these DI instruments, the controller and microscope have spearate manuals.
JPK (now a part of Bruker)
- Nanowizard User Manual - This .pdf file is the user manual for JPK (now Bruker) Nanowizard bio-AFM instruments. THis covers the version 6.0 software. As far as I know this means instruments up to Nanowizard 4. Covers hardware, control software and analysis functions.
Molecular Imaging/Agilent/Keysight
- Agilent 5500 User Guide revB - This .pdf is the user guide for the Agilent 5500 microscope, which I think used to be called the PicoPlus before it becasme an Agilent instrument
Other Documents
A Practical Guide to Scanning Probe Microscopy - This is a .pdf file of the SPM practical guide, which I beleive was first made by TopoMetrix, this is the Thermomicroscopes version. This is a very old document, but contains a lot of helpful information for AFM users, still. Not an instrument manual, it's a simple guide to the general principles of how an AFM works. If you read this and want something more in-depth, I reccomend this book!
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Frequently Asked Questions about Atomic Force Microscopy
by Peter Eaton
This FAQ was originally created for clients of the AFM, i.e. those whose samples I scan.
However, it's grown a lot, and should also answer many questions of people planning to use the AFM themselves, or researching the technique. Its contents include a description of AFM suitability to various samples, sample preparation, tips for scanning and data processing, and a short bibliography. There is also a guide to recognising artifacts in AFM.
All the material here is discussed in greater detail in the book "Atomic Force Microscopy".
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